Gallery of Structures Solved in the CingolaniLab
![](https://www.cingolanilab.org/files/2024/01/HBV-importins-15fa7221c4f29c29.jpg)
Hepatitis B virus (HBV) nucleocapsid bound to Importins
PDB entries (Cryo-EM): 8G5V, 8G8Y, 8G6V, and 8GCN
Maps: EMD-29756, EMD-29858, and EMD-29785
Yang et al., Science Adv., 2024
![](https://www.cingolanilab.org/files/2024/01/P22-virion-86867ca9b10c8b8c.jpg)
Salmonella-phage P22 mature virion
PDB entries (Cryo-EM): 8TVU, 8TVR, 8U10, 8U11, and 8U1O,
Maps: EMD-41651, EMD-41649, EMD-41791, EMD-41792, and EMD-41819
Iglesias et al., J. Mol Biol., 2023
![Cryo-EM Reconstruction of Pseudomonas-phage E217](https://www.cingolanilab.org/files/2024/01/Pseudomonas-virus-E217-5442374a268cf69e.jpg)
Pseudomonas-phage E217
PDB entries (Cryo-EM): 8FRS, 8FVH, 8FUV, 8FVG, and 8EON,
Maps: EMD-29406, EMD-29487, EMD-29481, EMD-29486, and EMD-28405
Li et al., Nature Commun., 2023
![Cryo-EM reconstruction of Shigella phage Sf6](https://www.cingolanilab.org/files/2024/01/Shigella-virus-Sf6-f111916188b81a9f.jpg)
Shigella-virus Sf6
PDB entries (Cryo-EM): 7SFS, 7SG7, 7SPU, 7SP4, and 7UKJ
Maps: EMD-25101, EMD-25106, EMD-23372, EMD-25365, and EMD-26582
Li et al., Science Adv., 2022
![X-ray structure TDP-43 NLS bound to importin a1](https://www.cingolanilab.org/files/2024/01/Human-TDP-43-NLS-bound-to-importin-a1-63fcef6eab77d625.jpg)
TDP-43 NLS bound to importin α1
PDB entry (X-ray): 7N9H
Doll et al., Cell Rep., 2022
![Cryo-EM reconstruction of Pseudomonas-phage E217 TerS](https://www.cingolanilab.org/files/2024/01/Pseudomonas-E217-TerS-bb647bba36aec9fd.jpg)
Pseudomonas-phage E217 TerS
PDB entries (Cryo-EM): 8DKR and 7UXE
Map: EMD-26858
Lokareddy et al., J Mol Biol., 2022
![Cryo-EM reconstruction of Pseudomonas-phage PaP3 Portal Protein](https://www.cingolanilab.org/files/2024/01/Pseudomonas-PaP3-Portal-Protein-f9d9a17010ea68d3.jpg)
Pseudomonas-phage PaP3 Portal Protein
PDB entries (Cryo-EM): 7SXK, 7SYA, 7SZ4 and 7SZ6
Maps: EMD- 25500, 25521, 25560, 25562, and 25572
Chun-Feng David Hou et al., J Mol Biol., 2022
![X-ray and SAXS structures of NF-kB p50/p65 bound to importin a3](https://www.cingolanilab.org/files/2024/01/NF-KB_p65-p50-Human-Imp-a3-4ebed4e06f3e09c1.jpg)
NF-kB p65/p50-Imp a3 Complex
PDB entries (X-ray/SAXS): 7LFC, 7LF4, 7LEU, 7LEQ, and 7LET
Florio et al., Nature Commun, 2022
![X-ray Structure of COX-1 bound to inhibitor](https://www.cingolanilab.org/files/2024/01/Synthetic-COX-1-Inhibitor-509c033ec55076a9.jpg)
Synthetic COX-1 Inhibitor
PDB entry (X-ray): 7JXT
Friedrich et al., Adv Sci., 2021
![Cryo-EM reconstruction of T7 gp15-gp16 complex](https://www.cingolanilab.org/files/2024/01/DNA-Ejectosome-Structures-d1410afa61c5bf6f.jpg)
Cryo-EM structure of the T7 DNA-Ejectosome
PDB entry (Cryo-EM): 7K5C. Map: EMD-22680
Swanson et al., Molecular Cell, 2021
![X-ray structure of Fab4 bound to TerL (1-31)](https://www.cingolanilab.org/files/2024/01/Fab4-TerL-0be82b49a036e4a1.jpg)
Recombinant Fab4 bound to TerL (1-31)
PDB entries (X-ray): 6VI2, 6VI1, 6XMI
Lokareddy et al., Acta D, 2020
![X-ray structure of Pseudomonas-phage PaP3](https://www.cingolanilab.org/files/2024/01/Pseudomonas-PaP3-TerS-b8c0f8218ba098c1.jpg)
Pseudomonas-phage PaP3 TerS
PDB entry (X-ray/SAXS): 6W7T
Niazi et al., Nucleic Acid Research, 2020
![X-ray structure of Chlamydia SNARE-like protein IncA](https://www.cingolanilab.org/files/2024/01/Chlamydia-IncA1-16b52a78fbbfceee.jpg)
Chlamydia SNARE-like Protein IncA
PDB entries (X-ray): 6E7E, 6E6A
Cingolani et al., Nature Commun., 2019
![X-ray Structure of Mycobacterium DPP1](https://www.cingolanilab.org/files/2024/01/Mycobacterium-DPP1-89e1929d9296f1bf-776x1024.jpg)
Mycobacterium Tuberculosis Heme Transporter DppA
PDB entries (X-ray): 6E3D, 6E4D
Mitra et al., Nature Commun., 2019
![X-ray and SAXS structures of S. cerevisiae Inositol Phosphatase Siw14](https://www.cingolanilab.org/files/2024/01/Siw14-Phosphatase-46681bd21026a771.jpg)
Saccharomyces cerevisiae Inositol Phosphatase Siw14
PDB entry (X-ray/SAXS): 6E3B
Florio et al., Biochemistry, 2019
![X-ray and SAXS structure of human and yeast RCC1 bound to importin a1/Kap60](https://www.cingolanilab.org/files/2024/01/Human-Imp-a3-RCC1-2-5494fe35bfa70208.jpg)
RCC1 bound to importin a3 and Kap60
PDB entries (X-ray/SAXS): 5TBK, 5T94
Sankhala et al., Nature Commun., 2017
![X-ray structure of COX-1 bound to Mofezolac](https://www.cingolanilab.org/files/2024/01/COX-1-Mofezolac-d8b2bfb373600f95.jpg)
Inhibition of COX-1 by Mofezolac and P6
PDB entries (X-ray): 5WBE, 5U6X
Cingolani et al., Eur. J. Med. Chem., 2017
![X-ray structure of P22 ProCapsid Portal Protein](https://www.cingolanilab.org/files/2024/01/Salmonella-P22-Portal_Procapsid-Conformation-4704c2863ad67aba.jpg)
Immature ProCapsid Portal Protein
PDB entries (X-ray): 5JJ3, 5JJ1
Lokareddy et al., Nature Commun., 2017
![Pom121 NLS bound to importin α](https://www.cingolanilab.org/files/2024/01/Human-Importin-a1-Pom121-NLS-56cccf0e6ef59b14.jpg)
Pom121 NLS bound to importin α
PDB entry (X-ray): 4YI0
Kralt et al., Mol. Cell. Bio., 2015
![Shigella-Sf6-TailNeedles](https://www.cingolanilab.org/files/2024/01/Shigella-Sf6-TailNeedles-20ae3973c6fc3e5f.jpg)
HK620 and P22 Tail Needles at different pHs
PDB entries (X-ray): 4ZKP, 4ZKU, 4ZXQ, 5BU5, 5BVZ, 5BU8
Bhardwaj et al., J. Biol. Chemistry, 2016
![Yeast-Importin-a1-Heh2-sNLS](https://www.cingolanilab.org/files/2024/01/Yeast-Importin-a1-Heh2-sNLS-ce45750f47ab8a92.jpg)
Heh1 and Heh2 membrane protein NLSs
PDB entries (X-ray): 4PVZ, 4XZR
Lokareddy et al., Structure, 2015
![Mycobacterium tubercolosis TNT-IFT](https://www.cingolanilab.org/files/2024/01/Mycobacterium-TNT-IFT-ca0cb919ffa5d4be.jpg)
Mycobacterium Tuberculosis TNT-IFT complex
PDB entry (X-ray): 4QLP
Sun et al., Nature Struct. Mol. Biol., 2015
![Importin α1, 3, 7 bound to Influenza](https://www.cingolanilab.org/files/2024/01/Importins-Influenza-Pb214-ee3bcdc0dbbcf12d.jpg)
Importin α1, 3, 7 bound to Influenza PB2
PDB entries (X-ray): 4UAD, 4UAE, 4UAF
Pumroy et al., Structure, 2015
![Phosphatase domain of Human Laforin](https://www.cingolanilab.org/files/2024/01/Human-Laforin-DSP-d1b64bbbe972342a.jpg)
Phosphatase domain of Human Laforin
PDB entry (X-ray): 4R30
Sankhala et al., J. Biol. Chemistry, 2015
![Human RNA Phosphatase PIR1](https://www.cingolanilab.org/files/2024/01/PIR1-DSP-5c2b6c58df88d62c.jpg)
Human RNA Phosphatase PIR1
PDB entries (X-ray): 4MBB, 4NYH
Sankhala et al., Biochemistry, 2014
![Human p53-phosphatase DUSP26](https://www.cingolanilab.org/files/2024/01/Human-DUSP26-DSP-fdbd80628dabb781.jpg)
Human p53-phosphatase DUSP26
PDB entry (X-ray): 4HRF
Lokareddy et al., Biochemistry, 2013
![P22 Headful Packaging Nuclease](https://www.cingolanilab.org/files/2024/01/Salmonella-P22-TerL-nuclease-54ee1c1f9220faa1.jpg)
P22 Headful Packaging Nuclease
PDB entry (X-ray): 4DKW
Roy et al., J. Biol. Chemistry, 2012
![Human-Importin-a5-Nup50](https://www.cingolanilab.org/files/2024/01/Human-Importin-a5-Nup50-ed016a0a2808345d.jpg)
Human Importin α5 bound to Nup50
PDB entry (X-ray): 3TJ3
Ruth et al., J. Biol. Chemistry, 2012
![P22 nonameric Small terminase subunit](https://www.cingolanilab.org/files/2024/01/Salmonella-P22-TerS-48d6f883e1cb92e7.jpg)
P22 nonameric Small terminase subunit
PDB entry (X-ray): 3P9A
Roy et al., Structure, 2012
![Importin α1 bound to PLSCR4 minimal NLS](https://www.cingolanilab.org/files/2024/01/Human-Importin-a1-PLSCR4-NLS-adc96b7d590fd50d.jpg)
Importin α1 bound to PLSCR4 minimal NLS
PDB entry (X-ray): 3Q5U
Lott et al., J. Biol. Chemistry, 2011
![Structure of phage Sf6 Tail Needle Knob](https://www.cingolanilab.org/files/2024/01/Shigella-Sf6-TailKnob-61da6012c901cf0d.jpg)
Structure of phage Sf6 Tail Needle Knob
PDB entries (X-ray): 3RWN, 4K6B
Bhardwaj et al., J. Biol. Chemistry, 2011
![E.coli F1 ATPase inhibited by subunit ε](https://www.cingolanilab.org/files/2024/01/E.Coli-F1-ATPase-665904aae0ad25f0.jpg)
E.coli F1 ATPase inhibited by subunit ε
PDB entry (X-ray): 3OAA
Cingolani and Duncan, Nature Struct. Mol. Biol., 2011
![Full length dodecameric P22 Portal Protein PDB entry: 3LJ5 Olia](https://www.cingolanilab.org/files/2024/01/Salmonella-P22-FullLength-Portal-519821ead6b78d60.jpg)
Full-length dodecameric P22 Portal Protein
PDB entry (X-ray): 3LJ5
Olia et al., Nature Struct. Mol. Biol., 2011
![P22 Portal Protein Core bound to twelve GP4s PDB entries: 1VT0,](https://www.cingolanilab.org/files/2024/01/Salmonella-P22-Portal-Core-Gp4-d0e3591947339b78.jpg)
P22 Portal Protein Core bound to twelve GP4s
PDB entries (X-ray): 1VT0, 3LJ4
Olia et al., Nature Struct. Mol. Biol., 2011
![Human-Importin-Beta Open and Close conformation](https://www.cingolanilab.org/files/2024/01/Human-Importin-Beta_Open-Close_conformation-194618cff11bb7f1.jpg)
Open and closed Importin β bound to sIBB
PDB entry (X-ray): 3LWW
Bhardwaj et al., Biochemistry, 2010
![Barnase-permutant](https://www.cingolanilab.org/files/2024/01/Barnase-permutant-c18c3d10ceb52b55.jpg)
A circular permutant of Barnase
PDB entry (X-ray): 3DA7
Butler et al., Biochemistry, 2009
![VacciniaVirus-VH1-phosphatase](https://www.cingolanilab.org/files/2024/01/VacciniaVirus-VH1-phosphatase-8d6c39172d53651e.jpg)
Vaccinia Virus Dual Specificity Phosphatase
PDB entries (X-ray/SAXS): 3CM3, 2RF6
Koksal et al., J. Biol. Chemistry, 2009
![Phage P22 Tail Needle Gp26](https://www.cingolanilab.org/files/2024/01/Salmonella-P22-TailNeedle-gp26-9c36848e0d9465d5.jpg)
Phage P22 Tail Needle Gp26
PDB entries (X-ray): 2POH, 3C9I
Olia et al., Nature Struct. Mol. Biol., 2007
![Importin beta-sIBB](https://www.cingolanilab.org/files/2024/01/Importin-beta-sIBB-5ec88b531e0e3a2c.jpg)
Importin β bound to snurportin IBB-domain
PDB entries (X-ray): 2P8Q, 2Q5D
Mitrousis et al., J. Biol. Chemistry, 2008
![Mammalian Importin α bound to PLSCR1 NLS](https://www.cingolanilab.org/files/2024/01/Human-Importin-a1-PLSCR1-NLS-751c788a0bfb2dec.jpg)
Mammalian Importin α bound to PLSCR1 NLS
PDB entry (X-ray): 1Y2A
Chen et al., J. Biol. Chemistry, 2005
![Human Importin Beta bound to PTHrP](https://www.cingolanilab.org/files/2024/01/Human-Importin-Beta_PTHrP-169820d2f2c3450b.jpg)
Importin β HEATs (1-11) bound to the PTHrP
PDB entry (X-ray): 1M5N
Cingolani et al., Molecular Cell, 2002
![Human-Importin-Beta_IBB](https://www.cingolanilab.org/files/2024/01/Human-Importin-Beta_IBB-3e4a59052a1bdfa2.jpg)
Human Importin β bound to the IBB-domain
PDB entries (X-ray): 1QGK, 1QGR
Cingolani et al., Nature, 1999