Publications

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2012

Roy, A. and Cingolani, G. (2012) Structure of P22 headful packaging nuclease. Submitted.

Pumroy, R.A., Nardozzi, J., Hart, D.J., Root, M.J. and Cingolani, G. (2012)Nup50 stabilizes the closed conformation of arm 10 in importin a5. J Biol Chem. 287(3):2022-31.

Roy, A., Bhardwaj, A., Lander, G.C. and Cingolani, G. (2011) Atomic structure of a nonameric small terminase subunit: implication for DNA recognition and assembly of a viral genome-packaging motor. Submitted.

2011

Lott, K., Bhardwaj, A., Sims, P. and Cingolani, G. (2011) A minimal Nuclear Localization Signal in human phospholipid Scramblase 4 that binds only the minor binding site in importin a. J Biol Chem. 286(32):28160-9.

Bhardwaj, A., Molineux, J.I., Casjens, S.R. and Cingolani, G. (2011) Atomic Structure of Sf6 Tail Needle Knob. J Biol Chem. 286(35):30867-77.

Cingolani, G. and Duncan, T.M. (2011) Structure of the ATP synthase catalytic complex (F1) from Escherichia coli in an auto-inhibited conformation. Nature Struc Mol Biol. 18(6):701-7.

Olia, A.S, Prevelige Jr., P.E., Johnson, J.E. and Cingolani, G. (2011) Three-dimensional structure of a viral genome-delivery portal vertex. Nature Struc Mol Biol. 18(5):597-603.

Jinghua, T., Lander, G., Olia, A.S, Prevelige Jr., P.E., Cingolani, G., Casjens, S., Baker, T.S. and Johnson, J.E. (2011) Peering down the barrel of a bacteriophage portal – the genome packaging and release valve in P22. Structure. 19(4):496-502.

Koksal, A. and Cingolani, G. (2011) Dimerization of Vaccinia virus VH1 is essential for dephosphorylation of STAT1 at tyrosine 701. J Biol Chem. 286(16):14373-82.

Roy, A., Bhardwaj, A., and Cingolani, G. (2011) Crystallization of the Nonameric Small Terminase Subunit of Bacteriophage P22. Acta Cryst. F. 67, 104-110.

Lott, K. and Cingolani, G. (2011) The IBB-domain as a master regulator of nucleocytoplasmic transport. Biochim Biophys Acta. 1813(9):1578-92.



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2010

Nardozzi, J., Lott, K. and Cingolani, G. (2010) Phosphorylation meets Nuclear Import: a review. Cell Communication and Signaling. 8(1):32.

Nardozzi, J., Wenta, N., Yasuhara, N., Vinkemeier, U. and Cingolani, G. (2010) Molecular basis for the recognition of phosphorylated STAT1 by importin a5. J. Mol. Biol. 402(1):83-100.

Bhardwaj, A. and Cingolani, G. (2010) Conformational selection in the recognition of snurportin IBB-domain by importin b. Biochemistry 49(24):5042-7.

Lott, K., Bhardwaj, A., Mitrousis, G., Pante, N., and Cingolani, G. (2010) The importin beta binding domain modulates avidity of importin b for the nuclear pore complex. J. Biol. Chem. 285(18):13769-80.



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2009

Bhardwaj, A., Walker-Kopp, N., Casjens, S.R., and Cingolani, G. (2009) An evolutionarily conserved family of virion tail needles related to bacteriophage P22 gp26: correlation between structural stability and length of the a-helical trimeric coiled-coil. J. Mol. Biol. 391(1):227-45.

Mitrea, D.M., Butler, J.S., Mitrousis, G., Cingolani, G. and Loh, S. N. (2009). Structural and Thermodynamic Analysis of Conformationally-Strained Circular Permutants of Barnase. Biochemistry. 48(15):3497-507.

Koksal, A., Nardozzi, J., and Cingolani, G. (2009) Dimeric quaternary structure of the prototypical dual specificity phosphatase VH1. J. Biol. Chem. 284(15):10129-37.

Olia, S.A., Casjens, S., and Cingolani, G. (2009) Structural Plasticity of the phage P22 tail-needle gp26 probed with Xenon Gas. Protein Science. 18(3):537-548.



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2008

Olia, S.A., and Cingolani, G. (2008) A shifty stop for a hairy tail. Mol. Microbiology, 70(3):549-53.

Bhardwaj, A., Walker-Kopp N, Wilkens, S., and Cingolani, G. (2008) Foldon-guided self-assembly of ultra stable protein fibers. Protein Science 17(9):1475-85.

Lorenzen, K., Olia, S.A., Uetrecht, C., Cingolani, G., and Heck, A.J.R. (2008). Determination of stoichiometry and conformational changes in the first step of the P22 tail assembly. J. Mol. Biol. 379(2):385-96.

Mitrousis, G., Olia, S.A., Walker and Cingolani, G. (2008). Molecular basis for the recognition of snurportin 1 by importin b. J. Biol. Chem. 283(12):7877-84.

Zheng, H., Olia, S.A., Hankins, M., Andrews, S., Cingolani, G., and Gonen, T, (2008). A conformational switch in bacteriophage P22 portal protein primes genome injection. Molecular Cell. 29(3), 376-383.



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2007

Olia, S.A., Casjens, S. and Cingolani, G. (2007) Structure of Phage P22 Cell Envelope Penetrating Needle. Nature Struc Mol Biol. 14(12), 1221-1226.

Olia, S.A., Bhardwaj, A., Joss, L., Casjens, S., and Cingolani, G. (2007) The role of gene 10 protein in the hierarchical assembly of the bacteriophage P22 portal vertex structure. Biochemistry. 46(30), 8776-8784.

Bhardwaj, A., Olia, S.A., Walker-Kopp N, and Cingolani, G. (2007) Domain organization and polarity of tail needle gp26 in the portal vertex structure of bacteriophage P22. J. Mol. Biol. 371(2), 374-87.



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2006

Olia, A., Al-Bassam, J., Winn-Stapley, D.A., Joss, L., Casjens, S., and Cingolani, G. (2006) Binding induced stabilization and oligomerization of portal closure factor gp4 in the assembly pathway of phage P22 tail. J. Mol. Biol. 363, 558–576.

Cingolani, G., Andrews, D., and Casjens, S. (2006) Crystallogenesis of bacteriophage P22 tail accessory factor gp26 at acidic and neutral pH. Acta Cryst. F 62, 477–482.

Taylor, D.J., Speir, J.A., Reddy, V., Cingolani, G., Pringle, F.M., Ball, L.A. and Johnson, J.E. (2006) Preliminary X-ray characterization of authentic providence virus and attempts to express its coat protein gene in recombinant baculovirus. Arch Virol. 151(1):155-65.



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2005

Andrews D, Butler JS, Al-Bassam J, Joss L, Winn-Stapley DA, Casjens S, and Cingolani, G. (2005) Bacteriophage P22 Tail Accessory Factor GP26 Is a Long Triple-stranded Coiled-coil. J. Biol. Chem. 280(7): 5929-33.

Chen MH, Ben-Efraim I, Mitrousis G, Walker-Kopp N, Sims PJ, and Cingolani, G. (2005) Phospholipid scramblase 1 (PLSCR1) contains a non-classical nuclear localization signal with unique binding site in importin a. J. Biol. Chem. 280(11):10599-606.

Tang L., Marion W., Cingolani, G., Prevelige P.E. Jr., and Johnson J.E. (2005) Three-dimensional structure of the bacteriophage P22 tail machine EMBO J. 24(12):2087-95.



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2004 - 2001

Wodrich, H., Guan, T., Cingolani, G., von Seggern, D., Nemerow, G., and Gerace, L. (2003). Switch from capsid protein import to adenovirus assembly by cleavage of nuclear transport signals. EMBO J. 22 (23): 6150-6162.

Bednenko, J., Cingolani, G., and Gerace, L. (2003). Importin beta contains a COOH-terminal nucleoporin binding region important for nuclear import. J. Cell Biol. 162(3): 391-401.

Koerner, C., Guan, T., Gerace, L. and Cingolani, G. (2003). Synergistic effects of silent and hot spot mutations in importin b reveals a dynamic mechanism for recognition of a nuclear localization signal. J. Biol. Chem. 278 (18): 16216-16221.

Cingolani, G., and Gerace, L. (2003). Molecular basis of nucleocytoplasmic transport. Handbook of Cell Signaling, Academic Press, Vol. 3(330): 419-429.

Bednenko, J., Cingolani, G., and Gerace, L. (2003). Nucleocytoplasmic transport: navigating the channel (NPC). Traffic, 4(3): 27-135.

Cingolani, G., Bednenko, J., Gillespie, M., and Gerace, L. (2002). Molecular basis for the recognition of a non-classical nuclear localization signal (NLS) by importin b. Molecular Cell, 10: 1345-1353.

Cingolani, G., Moore, S., Privilege, P., and Johnson, J. (2002). Preliminary crystallographic analysis of the bacteriophage P22 portal protein. J. Struc. Biology, 139: 46-54.



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2000 - 1997

Cingolani, G., Lashuel, H., Gerace, L., and Mueller, C.W. (2000). Nuclear import factors importin a and importin b undergo mutually induced conformational changes upon association. FEBS Lett., 484(3): 291-298.

Chook, Y.M, Cingolani, G., Conti, E., Stewart, M., Vetter, I., and Wittinghofer, A. (1999). Pictures in cell biology. Structures of nuclear-transport components. Trends Cell Biol., 9(8): 310-311

Cingolani, G., Petosa, C., Weis, K., and Mueller, C.W. (1999). Structure of importin b bound to the IBB domain of importin a. Nature, 399(6733): 221-229.

Cingolani, G., Capaccio, L., D'Elia, D., and Gadaleta, G. (1997). In organello footprinting analysis of rat mitochondrial DNA: protein interaction upstream of the Ori-L. Biochem Biophys Res Commun, 231(3): 856-860.



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